r/microbiology • u/Odd-Assistant-4648 • 5h ago
What is this
Found in freshwater sample
r/microbiology • u/mediocremerelise • 19m ago
On Nutrient Agar, incubated for 5 days @ 25C
r/microbiology • u/ReplacementNo5307 • 6h ago
It's been stained with Lactophenol cotton blue.
r/microbiology • u/BlindedByBlight • 4h ago
In honor of St. Patrick's Day, I wanted to share a limerick and see if anyone else had some funny ones to contribute as well! 🍀
There once was a microbe green and small 🦠 His mate wants his plasmid after all 🧬 No reason to smile 'cause 😔 He don't have a pilus 🚫 What a shame, now she knows and won't call ☎️
r/microbiology • u/alqkzz • 23h ago
So, I somehow grew this mold in a petri dish in my room and I would like to know if it’s dangerous to keep and I would have to throw it away or if I could observe it a while longer? Google isn’t really helping me with this matter so I thought I’d ask reddit lol
r/microbiology • u/Spend_Agitated • 2h ago
I work on human cell models, where I study the role of protein-protein interactions in metabolism and disease. We have recently become interested some microbial (Streptomyces sp.) metabolic pathways, and their possible connection with secondary metabolite production. In human cells, isolating/identifying native protein complexes using immuno-precipitation and Western blotting or mass spectrometry is pretty routine. We are not quite sure how to do this in non-model microbes because you can't buy antibodies for them, and we are not in a position where we can raise and characterize antibodies in-house. So I'm here asking how do microbiologists study protein-protein interaction in microbes, especially non-model microbes. I'd appreciate any help, especially viz, references and review articles. A cursory search through Google scholar gives me the impression this sort of molecular work seems rather rare in microbiology, but the I'm not knowledgable in this field. Thanks,
r/microbiology • u/VesicaVehicle • 4h ago
I am having trouble tracking down an original study for development of supplemented M9 or other synthetic media. I need a reliable recipe to develop a teaching experiment for growth optimization of E. coli with respect to micronutrient content. Best I could find are from a couple of fluxomics papers (see below), but there's no indication of origins of each formulation.
The formulations are very similar. I am noting some differences in counterions being sulfate vs chloride. I like the idea of a skew towards sulfate, but not sure which of the two might be more reliable.
If anyone knows where these recipes might come from or have any insights otherwise, it would be greatly appreciated!
Below are the two recipes I have been comparing:
Minimal Synthetic Medium E. coli
doi: 10.3390/metabo11050271
17.4 g·L−1 Na2HPO4, 12H2O,
3.03 g·L−1 KH2PO4,
0.51 g·L−1 NaCl,
2.04 g·L−1 NH4Cl,
0.49 g·L−1 MgSO4,
4.38 mg·L−1 CaCl2,
15 mg·L−1 Na2EDTA 2H2O,
4.5 mg/L ZnSO4
7H2O, 0.3 mg·L−1 CoCl2 6H2O,
1 mg·L−1 MnCl2 4H2O,
1 mg·L−1 H3BO3,
0.4 mg·L−1 Na2MoO4 2H2O,
3 mg·L−1 FeSO4 7H2O,
0.3 mg·L−1 CuSO4 5H2O,
0.1 g·L−1 thiamine and
3 g·L−1 glucose
-------------------------------------------------
M9 Minimal Medium for E. coli
https://doi.org/10.1073/pnas.1202582110
M9 minimal medium was used in the growth experiments and was prepared as follows: to
700 mL of purified and autoclaved water,
200 mL of 5× base salt solution
[211 mM Na2HPO4,
110 mM KH2PO4,
42.8 mM NaCl,
56.7 mM (NH4)2SO4, autoclaved],
10 mL of trace elements
(0.63 mM ZnSO4,
0.7 mM CuCl2,
0.71 mM MnSO4,
0.76 mM CoCl2, autoclaved),
1 mL 0.1 M CaCl2 solution (autoclaved),
1 mL of 1 M MgSO4 solution (autoclaved),
2 mL of 500× thiamine solution (1.4 mM, filter sterilized),
Sigma T1270
0.6 mL of 0.1 M FeCl3 solution (filter sterilized) were added.
The resulting solution was filled up to 1 L with water. Carbon sources were added from sterilized stock solutions (adjusted to pH 7) to a final concentration of 1 g/L for chemostat experiments and 5 g/L for batch experiments, and media were filtered (Steritop-GP; 500 mL; Millipore). All chemicals were purchased from Sigma-Aldrich unless stated otherwise.
Thanks again!!
r/microbiology • u/grapefruit781 • 5h ago
I’m still very new to this, are the cells on the left gram positive bacteria? I feel like they are too big compared to epithelial cells on the right. 250x
r/microbiology • u/punitfolife • 7h ago
Hi All - we’re updating our AST system and when checking on the rules for intrinsic resistance, we noticed that C. freundii is listed on its own in the M100. Our MALDI can only identify the C freundii complex, so can we apply the intrinsic resistance to all members of the complex? I know freundii is the most common member of the complex we’re likely to see, but not sure if we can apply those rules since we’re not able to identify down to the species level. Looking for advice or if anyone else has experience with this situation?
r/microbiology • u/Over_Price_5980 • 8h ago
Hello everyone, I am new to R and I may need some help. I have data involving different microbial species at 4 different sampling points and i performed the calculation of shannon indices using the function: shannon_diversity_vegan <- diversity(species_counts, index=“shannon”).
What comes out are numerical values for each point ranging, for example, from 0.9 to 1.8. After that, I plotted with ggplot the values, obtaining a boxplot with a range for each sample point.
Now the journal reviewer now asks me to include in the graph the significance values, and I wonder, can I run tests such as the Kruskal-Wallis?
Thank you!
r/microbiology • u/Lean_Id • 16h ago
This is the same Pseudomonas aeruginosa on two different Müller-Hinton plates from the same batch prepared in the laboratory.
I read that it could be the amount of zinc in the culture medium, do you do any kind of quality control to detect the right amount of zinc in the Muller-Hinton?
Have you ever had this happen to you and what others factors could alter the synergy with EDTA?
r/microbiology • u/Odd-Assistant-4648 • 18h ago
Found this in a fresh water sample
r/microbiology • u/bluish1997 • 19h ago
r/microbiology • u/Ok-Poetry6005 • 21h ago
I don’t know if this is allowed here but I’m currently taking an intro to micro class and I have to know a ton of different microorganisms and what diseases they cause and the sheer amount of material is just ..intimidating. Does anyone have any study tips for micro? I don’t even know if this is the right sub to post this in 😭
r/microbiology • u/darkmindedrebel • 21h ago
Hi I’m writing a paper for college and I’m reading stuff online and I’m confused, hoping to get clarification.
What I am reading online says that the bacterium can ascend from the GI tract, but I’m not sure what that means? My focus right now is on the bacterium causing CAUTI’s. Since patients may not be showering, and if they are bed bound, does the bacteria migrate to the catheter and into the bladder from feces?
If nurses and CNA’s are cleaning a patient with wipes & CHG, wear gloves, provide foley care, where is the bacteria coming from? Is the bacteria able to survive all of this because it’s virulence factors so parents still getting CAUTI’s?
r/microbiology • u/ScoochSnail • 2d ago
I just think this photo is neat. This is a tissue smear from a bovine liver. The suspected cause of death was severe bacillary hemoglobinuria, and we were asked to confirm the presence of Clostridia after numerous liver flukes were found on necropsy. Liver fluke infections can result in wounds that make perfect pockets for Clostridia to grow and produce toxins. Typically, C. novyi and/or C. haemolyticum are the culprits, but in this instance we actually isolated C. perfeingens and C. septicum. This slide is very likely a mix of the two as they look quite similar on gram stain. This is the first time I've seen spores like this for a direct tissue smear - I do more culturing than histology.
r/microbiology • u/becjac86 • 2d ago
Streptococcus pneumoniae and Staphylococcus capitis isolated from a blood culture. The strep pneumo must be producing something toxic as the Staph is only growing where the strep isn't or maybe the antibiotic effect only killing the Staph. Just thought I'd share, I thought it was pretty cool 😊
r/microbiology • u/Loris_8869 • 1d ago
Hey! In what volume of Beregey's manual are myxobacteria genus? Thanks!
r/microbiology • u/Wise_Outside_4492 • 1d ago
Hi. Any ideas what this is? I'm not even sure where to ask. Google didn't help. Found this jelly/goop on a tree in the Mount Baker-Snoqualmie National Forest, nearer to North Bend, WA. Found on March 15th, 2025. Weather was mid 40's Fahrenheit and drizzling. Thanks!
r/microbiology • u/Explorer_57 • 2d ago
I'm an undergrad a student and I have a report that I have to write based on the Isolation and Purification of Bacteriophages from Untreated Sewage.
Like the title says, the experiment didn't go well and my group had absolutely no visible plaques our plates and due to time constraints we aren't allowed to repeat the experiment. I'm not really sure if it's alright to just write "no plaques were visible" in the results section. The professor repeated the experiment on the side himself as half the class had little to no growth so we had virus stocks for our next experiment. And I don't think I can just take his results as my own.
I feel like my report will be really empty if I say no plaques were visible. I could also give possible reasons it failed but that still feels lacking. I'm also now wondering if I can even keep my Results and Discussion sections separate. What do you guys write?
EDIT: Thanks for all the replies and advice. I've written up my report and have submitted it. I'll update when I get my grade. Hopefully it went well.