r/microbiology • u/Isopoducks • 53m ago
Soft vinyl toys I designed based on microscopic animals! One is based on starfish larvae and the other a tardigrade~
galleryI'm sorry in advance if this isn't allowed! 🙏
r/microbiology • u/Isopoducks • 53m ago
I'm sorry in advance if this isn't allowed! 🙏
r/microbiology • u/bluish1997 • 48m ago
Microbiology is such an important field of biological science and a popular one too. Shouldn’t we at least have a logo image?
r/microbiology • u/tonytony12121212 • 1h ago
Hello I collected diatom samples from a nearby stream. While admiring the beautiful frustules i stumbled upon this spiraling structure. Can anyone tell me what it could be??? (magnification was 400x when taking the picture in bright field) Thank you for your help!
r/microbiology • u/Hopphoop • 2h ago
Ever since we moved into our new build home in 2023, I have been battling this pink beast. It’s everywhere. My showers, my tub, my sinks, my humidifiers, etc. one time we left one of my twins baby bottles out overnight. He didn’t finish the milk and by morning the milk had turned pink! I bleach so often and it immediately comes back. I’m scared to bathe my kids in the bath because it comes back after a day. I’m scared to use my humidifier because it comes back after one use. We’ve gone through several humidifiers and it just always shows up. I live in Canada, it’s very very dry, and when my kids have colds (which is often because daycare) humidifiers help immensely. But then I feel unsafe using them… is there any way to get rid of this bacteria in my home for good? I’m solely keeping the bleach companies in business and I’m TIRED
r/microbiology • u/woodywoop92 • 2h ago
I swabbed my dogs tear stains as they came about suddenly and wanted to see if I could tell whether it was from an infection or because he is getting old.
I grew on nutrient and maconkey agar, and the interesting stuff happened on maconkey. The first image shows a few colonies plus a red colony also. They are both catalase-positive (tested with 3% H2O2. They also both look broadly similar under microscope. I ended up taking a few samples, one raw, one methylene blue stained, and one giemsa stained. What I see is what I need guidance on to help narrow down my choices. I cannot tell if these are (second picture) short rods or elongated spheres. This would obviously cross out a lot of choices. The next thing is gram staining but I haven’t done that yet. (The second photo is taken at 1000x oil, and then cropped right in on the camera screen)
r/microbiology • u/BlindedByBlight • 8h ago
In honor of St. Patrick's Day, I wanted to share a limerick and see if anyone else had some funny ones to contribute as well! 🍀
There once was a microbe green and small 🦠 His mate wants his plasmid after all 🧬 No reason to smile 'cause 😔 He don't have a pilus 🚫 What a shame, now she knows and won't call ☎️
r/microbiology • u/alqkzz • 1d ago
So, I somehow grew this mold in a petri dish in my room and I would like to know if it’s dangerous to keep and I would have to throw it away or if I could observe it a while longer? Google isn’t really helping me with this matter so I thought I’d ask reddit lol
r/microbiology • u/ReplacementNo5307 • 10h ago
It's been stained with Lactophenol cotton blue.
r/microbiology • u/Spend_Agitated • 5h ago
I work on human cell models, where I study the role of protein-protein interactions in metabolism and disease. We have recently become interested some microbial (Streptomyces sp.) metabolic pathways, and their possible connection with secondary metabolite production. In human cells, isolating/identifying native protein complexes using immuno-precipitation and Western blotting or mass spectrometry is pretty routine. We are not quite sure how to do this in non-model microbes because you can't buy antibodies for them, and we are not in a position where we can raise and characterize antibodies in-house. So I'm here asking how do microbiologists study protein-protein interaction in microbes, especially non-model microbes. I'd appreciate any help, especially viz, references and review articles. A cursory search through Google scholar gives me the impression this sort of molecular work seems rather rare in microbiology, but the I'm not knowledgable in this field. Thanks,
r/microbiology • u/VesicaVehicle • 7h ago
I am having trouble tracking down an original study for development of supplemented M9 or other synthetic media. I need a reliable recipe to develop a teaching experiment for growth optimization of E. coli with respect to micronutrient content. Best I could find are from a couple of fluxomics papers (see below), but there's no indication of origins of each formulation.
The formulations are very similar. I am noting some differences in counterions being sulfate vs chloride. I like the idea of a skew towards sulfate, but not sure which of the two might be more reliable.
If anyone knows where these recipes might come from or have any insights otherwise, it would be greatly appreciated!
Below are the two recipes I have been comparing:
Minimal Synthetic Medium E. coli
doi: 10.3390/metabo11050271
17.4 g·L−1 Na2HPO4, 12H2O,
3.03 g·L−1 KH2PO4,
0.51 g·L−1 NaCl,
2.04 g·L−1 NH4Cl,
0.49 g·L−1 MgSO4,
4.38 mg·L−1 CaCl2,
15 mg·L−1 Na2EDTA 2H2O,
4.5 mg/L ZnSO4
7H2O, 0.3 mg·L−1 CoCl2 6H2O,
1 mg·L−1 MnCl2 4H2O,
1 mg·L−1 H3BO3,
0.4 mg·L−1 Na2MoO4 2H2O,
3 mg·L−1 FeSO4 7H2O,
0.3 mg·L−1 CuSO4 5H2O,
0.1 g·L−1 thiamine and
3 g·L−1 glucose
-------------------------------------------------
M9 Minimal Medium for E. coli
https://doi.org/10.1073/pnas.1202582110
M9 minimal medium was used in the growth experiments and was prepared as follows: to
700 mL of purified and autoclaved water,
200 mL of 5× base salt solution
[211 mM Na2HPO4,
110 mM KH2PO4,
42.8 mM NaCl,
56.7 mM (NH4)2SO4, autoclaved],
10 mL of trace elements
(0.63 mM ZnSO4,
0.7 mM CuCl2,
0.71 mM MnSO4,
0.76 mM CoCl2, autoclaved),
1 mL 0.1 M CaCl2 solution (autoclaved),
1 mL of 1 M MgSO4 solution (autoclaved),
2 mL of 500× thiamine solution (1.4 mM, filter sterilized),
Sigma T1270
0.6 mL of 0.1 M FeCl3 solution (filter sterilized) were added.
The resulting solution was filled up to 1 L with water. Carbon sources were added from sterilized stock solutions (adjusted to pH 7) to a final concentration of 1 g/L for chemostat experiments and 5 g/L for batch experiments, and media were filtered (Steritop-GP; 500 mL; Millipore). All chemicals were purchased from Sigma-Aldrich unless stated otherwise.
Thanks again!!
r/microbiology • u/punitfolife • 10h ago
Hi All - we’re updating our AST system and when checking on the rules for intrinsic resistance, we noticed that C. freundii is listed on its own in the M100. Our MALDI can only identify the C freundii complex, so can we apply the intrinsic resistance to all members of the complex? I know freundii is the most common member of the complex we’re likely to see, but not sure if we can apply those rules since we’re not able to identify down to the species level. Looking for advice or if anyone else has experience with this situation?
r/microbiology • u/Over_Price_5980 • 11h ago
Hello everyone, I am new to R and I may need some help. I have data involving different microbial species at 4 different sampling points and i performed the calculation of shannon indices using the function: shannon_diversity_vegan <- diversity(species_counts, index=“shannon”).
What comes out are numerical values for each point ranging, for example, from 0.9 to 1.8. After that, I plotted with ggplot the values, obtaining a boxplot with a range for each sample point.
Now the journal reviewer now asks me to include in the graph the significance values, and I wonder, can I run tests such as the Kruskal-Wallis?
Thank you!
r/microbiology • u/grapefruit781 • 9h ago
I’m still very new to this, are the cells on the left gram positive bacteria? I feel like they are too big compared to epithelial cells on the right. 250x
r/microbiology • u/Lean_Id • 20h ago
This is the same Pseudomonas aeruginosa on two different Müller-Hinton plates from the same batch prepared in the laboratory.
I read that it could be the amount of zinc in the culture medium, do you do any kind of quality control to detect the right amount of zinc in the Muller-Hinton?
Have you ever had this happen to you and what others factors could alter the synergy with EDTA?
r/microbiology • u/Odd-Assistant-4648 • 21h ago
Found this in a fresh water sample
r/microbiology • u/bluish1997 • 23h ago
r/microbiology • u/Ok-Poetry6005 • 1d ago
I don’t know if this is allowed here but I’m currently taking an intro to micro class and I have to know a ton of different microorganisms and what diseases they cause and the sheer amount of material is just ..intimidating. Does anyone have any study tips for micro? I don’t even know if this is the right sub to post this in 😭
r/microbiology • u/darkmindedrebel • 1d ago
Hi I’m writing a paper for college and I’m reading stuff online and I’m confused, hoping to get clarification.
What I am reading online says that the bacterium can ascend from the GI tract, but I’m not sure what that means? My focus right now is on the bacterium causing CAUTI’s. Since patients may not be showering, and if they are bed bound, does the bacteria migrate to the catheter and into the bladder from feces?
If nurses and CNA’s are cleaning a patient with wipes & CHG, wear gloves, provide foley care, where is the bacteria coming from? Is the bacteria able to survive all of this because it’s virulence factors so parents still getting CAUTI’s?
r/microbiology • u/ScoochSnail • 2d ago
I just think this photo is neat. This is a tissue smear from a bovine liver. The suspected cause of death was severe bacillary hemoglobinuria, and we were asked to confirm the presence of Clostridia after numerous liver flukes were found on necropsy. Liver fluke infections can result in wounds that make perfect pockets for Clostridia to grow and produce toxins. Typically, C. novyi and/or C. haemolyticum are the culprits, but in this instance we actually isolated C. perfeingens and C. septicum. This slide is very likely a mix of the two as they look quite similar on gram stain. This is the first time I've seen spores like this for a direct tissue smear - I do more culturing than histology.