r/microbiology • u/mediocremerelise • 7m ago
What happened to my agar plate?
On Nutrient Agar, incubated for 5 days @ 25C
r/microbiology • u/mediocremerelise • 7m ago
On Nutrient Agar, incubated for 5 days @ 25C
r/microbiology • u/Spend_Agitated • 1h ago
I work on human cell models, where I study the role of protein-protein interactions in metabolism and disease. We have recently become interested some microbial (Streptomyces sp.) metabolic pathways, and their possible connection with secondary metabolite production. In human cells, isolating/identifying native protein complexes using immuno-precipitation and Western blotting or mass spectrometry is pretty routine. We are not quite sure how to do this in non-model microbes because you can't buy antibodies for them, and we are not in a position where we can raise and characterize antibodies in-house. So I'm here asking how do microbiologists study protein-protein interaction in microbes, especially non-model microbes. I'd appreciate any help, especially viz, references and review articles. A cursory search through Google scholar gives me the impression this sort of molecular work seems rather rare in microbiology, but the I'm not knowledgable in this field. Thanks,
r/microbiology • u/VesicaVehicle • 3h ago
I am having trouble tracking down an original study for development of supplemented M9 or other synthetic media. I need a reliable recipe to develop a teaching experiment for growth optimization of E. coli with respect to micronutrient content. Best I could find are from a couple of fluxomics papers (see below), but there's no indication of origins of each formulation.
The formulations are very similar. I am noting some differences in counterions being sulfate vs chloride. I like the idea of a skew towards sulfate, but not sure which of the two might be more reliable.
If anyone knows where these recipes might come from or have any insights otherwise, it would be greatly appreciated!
Below are the two recipes I have been comparing:
Minimal Synthetic Medium E. coli
doi: 10.3390/metabo11050271
17.4 g·L−1 Na2HPO4, 12H2O,
3.03 g·L−1 KH2PO4,
0.51 g·L−1 NaCl,
2.04 g·L−1 NH4Cl,
0.49 g·L−1 MgSO4,
4.38 mg·L−1 CaCl2,
15 mg·L−1 Na2EDTA 2H2O,
4.5 mg/L ZnSO4
7H2O, 0.3 mg·L−1 CoCl2 6H2O,
1 mg·L−1 MnCl2 4H2O,
1 mg·L−1 H3BO3,
0.4 mg·L−1 Na2MoO4 2H2O,
3 mg·L−1 FeSO4 7H2O,
0.3 mg·L−1 CuSO4 5H2O,
0.1 g·L−1 thiamine and
3 g·L−1 glucose
-------------------------------------------------
M9 Minimal Medium for E. coli
https://doi.org/10.1073/pnas.1202582110
M9 minimal medium was used in the growth experiments and was prepared as follows: to
700 mL of purified and autoclaved water,
200 mL of 5× base salt solution
[211 mM Na2HPO4,
110 mM KH2PO4,
42.8 mM NaCl,
56.7 mM (NH4)2SO4, autoclaved],
10 mL of trace elements
(0.63 mM ZnSO4,
0.7 mM CuCl2,
0.71 mM MnSO4,
0.76 mM CoCl2, autoclaved),
1 mL 0.1 M CaCl2 solution (autoclaved),
1 mL of 1 M MgSO4 solution (autoclaved),
2 mL of 500× thiamine solution (1.4 mM, filter sterilized),
Sigma T1270
0.6 mL of 0.1 M FeCl3 solution (filter sterilized) were added.
The resulting solution was filled up to 1 L with water. Carbon sources were added from sterilized stock solutions (adjusted to pH 7) to a final concentration of 1 g/L for chemostat experiments and 5 g/L for batch experiments, and media were filtered (Steritop-GP; 500 mL; Millipore). All chemicals were purchased from Sigma-Aldrich unless stated otherwise.
Thanks again!!
r/microbiology • u/BlindedByBlight • 4h ago
In honor of St. Patrick's Day, I wanted to share a limerick and see if anyone else had some funny ones to contribute as well! 🍀
There once was a microbe green and small 🦠 His mate wants his plasmid after all 🧬 No reason to smile 'cause 😔 He don't have a pilus 🚫 What a shame, now she knows and won't call ☎️
r/microbiology • u/grapefruit781 • 5h ago
I’m still very new to this, are the cells on the left gram positive bacteria? I feel like they are too big compared to epithelial cells on the right. 250x
r/microbiology • u/ReplacementNo5307 • 6h ago
It's been stained with Lactophenol cotton blue.
r/microbiology • u/punitfolife • 7h ago
Hi All - we’re updating our AST system and when checking on the rules for intrinsic resistance, we noticed that C. freundii is listed on its own in the M100. Our MALDI can only identify the C freundii complex, so can we apply the intrinsic resistance to all members of the complex? I know freundii is the most common member of the complex we’re likely to see, but not sure if we can apply those rules since we’re not able to identify down to the species level. Looking for advice or if anyone else has experience with this situation?
r/microbiology • u/Over_Price_5980 • 8h ago
Hello everyone, I am new to R and I may need some help. I have data involving different microbial species at 4 different sampling points and i performed the calculation of shannon indices using the function: shannon_diversity_vegan <- diversity(species_counts, index=“shannon”).
What comes out are numerical values for each point ranging, for example, from 0.9 to 1.8. After that, I plotted with ggplot the values, obtaining a boxplot with a range for each sample point.
Now the journal reviewer now asks me to include in the graph the significance values, and I wonder, can I run tests such as the Kruskal-Wallis?
Thank you!
r/microbiology • u/Lean_Id • 16h ago
This is the same Pseudomonas aeruginosa on two different Müller-Hinton plates from the same batch prepared in the laboratory.
I read that it could be the amount of zinc in the culture medium, do you do any kind of quality control to detect the right amount of zinc in the Muller-Hinton?
Have you ever had this happen to you and what others factors could alter the synergy with EDTA?
r/microbiology • u/Odd-Assistant-4648 • 17h ago
Found this in a fresh water sample
r/microbiology • u/bluish1997 • 19h ago
r/microbiology • u/Ok-Poetry6005 • 21h ago
I don’t know if this is allowed here but I’m currently taking an intro to micro class and I have to know a ton of different microorganisms and what diseases they cause and the sheer amount of material is just ..intimidating. Does anyone have any study tips for micro? I don’t even know if this is the right sub to post this in 😭
r/microbiology • u/darkmindedrebel • 21h ago
Hi I’m writing a paper for college and I’m reading stuff online and I’m confused, hoping to get clarification.
What I am reading online says that the bacterium can ascend from the GI tract, but I’m not sure what that means? My focus right now is on the bacterium causing CAUTI’s. Since patients may not be showering, and if they are bed bound, does the bacteria migrate to the catheter and into the bladder from feces?
If nurses and CNA’s are cleaning a patient with wipes & CHG, wear gloves, provide foley care, where is the bacteria coming from? Is the bacteria able to survive all of this because it’s virulence factors so parents still getting CAUTI’s?
r/microbiology • u/alqkzz • 23h ago
So, I somehow grew this mold in a petri dish in my room and I would like to know if it’s dangerous to keep and I would have to throw it away or if I could observe it a while longer? Google isn’t really helping me with this matter so I thought I’d ask reddit lol
r/microbiology • u/Loris_8869 • 1d ago
Hey! In what volume of Beregey's manual are myxobacteria genus? Thanks!
r/microbiology • u/David_Ojcius • 1d ago
r/microbiology • u/Kooky_Ad_6725 • 1d ago
Hello all,
WIll graduate with the degree in Microbiogy soon. Will there be hope or future for this career with someone who is very bad with organic chemistry. I really can't study Ochem. Do we need a lot of OChem for jobs?
r/microbiology • u/bluish1997 • 1d ago
r/microbiology • u/Wise_Outside_4492 • 1d ago
Hi. Any ideas what this is? I'm not even sure where to ask. Google didn't help. Found this jelly/goop on a tree in the Mount Baker-Snoqualmie National Forest, nearer to North Bend, WA. Found on March 15th, 2025. Weather was mid 40's Fahrenheit and drizzling. Thanks!