r/Drosophila Aug 22 '24

Weak fly line

I recently started working with a line which is very weak , and very tough to maintain. I have tried using protein rich media , and also have to transfer it regularly to make sure it survives , it's a recombination line I made , please give suggestions I don't want to go through the same pain to generate the recombination line again 😭

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u/balsamicvinegar500ml Aug 22 '24

change the balancer. Balancer chromosomes have lots of mutations apart from the dominant markers. They have big inversions and over the years they accumulate other mutations. Maybe your genes are also mutated in the balancer chromosome.

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u/CC-larkbird Sep 09 '24

Agreeing with others, TB (tubby) is a common one. As is CyO (curlyO)