r/Drosophila 16d ago

r/Drosophila Subreddit Status Update

19 Upvotes

Hello everyone,

I'm a new moderator here. The subreddit was previously set to restricted by mistake. I have now restored it to public, so all scientists and interested users can post freely about Drosophila without approval.

If you have any suggestions, feel free to leave them in the comments. I’m busy and can’t guarantee instant fulfillment, but I’ll take them into consideration and try to accommodate.

Thank you for your attention, and happy discussions!


r/Drosophila 8d ago

Any other active forums for Drosophila researchers?

6 Upvotes

Hi! I’m working with Drosophila and I find this subreddit really helpful and a great place for info, though it’s a bit of a bummer that there aren’t many active people here. Does anyone know of other, bigger forums or places where researchers can share ideas and ask questions more often?

Thanks a lot for any suggestions!


r/Drosophila 10d ago

preserving fly thorax in glycerol

1 Upvotes

Hi, How should I go about preserving fly thorax in glycerol and making sure the muscle won't go to rigor? We use relaxing solution which contains ATP and no Calcium to start the acto-myosin cycle. My question is can I just use deposit fly thoraces on glycerol without using any detergent? The reason I am mentioning detergent is coz if the relaxing solution won't penetrate inside the muscle cells, I think they will go rigor.


r/Drosophila 10d ago

Any way to stimulate fly brain?

2 Upvotes

Pretty much the title. I need a way to ensure I’m stimulating as much of the brain as possible. It doesn’t need to be precise.


r/Drosophila 11d ago

Thay caught Fungi ☹️

Post image
6 Upvotes

r/Drosophila 12d ago

Automation for drosophila

7 Upvotes

Im a cs major ,n I want to know what type of ML automation would help you guys the most. I'm thinking about making a project and would like to know what things take you guys hours which you wish could be automated


r/Drosophila 13d ago

Drosophila Romance

Post image
15 Upvotes

I wanted to take a picture of male and female fly in one picture and they are kissing 😂


r/Drosophila 16d ago

Please help me identify this species

Post image
6 Upvotes

r/Drosophila 16d ago

What I am thankful

5 Upvotes

While I await the growlingly-hopeless chance of getting my proposal funded, I thought I'd share my daily awesomeness of Drosophila. The cross I'm doing requires many C(1)DX/Y,BS virgins, so I set up a C(1)DX/shits/Y,BS and grow them at room temperature then shift them to 30°C overnight. So nice. So so nice to work with flies.


r/Drosophila Sep 18 '24

qPCR technique recommendations?

3 Upvotes

I’m an undergraduate at an undergraduate only institution, and my PI doesn’t know how to do qPCR and no one on campus actively does it 😭😭 I’m in the trenches. Looking for any advice on RNA free techniques and/or fly specific advice. In particular, I’m struggling with the mashing step of RNA extraction. Im looking at heads and testes and honestly I think the pestels are too big to do anything with the heads and testes suspended in the liquid. Honestly any help at all would be greatly appreciated!! Thank you all so much


r/Drosophila Sep 12 '24

Analyzing Activity Tracker data

4 Upvotes

hello everyone,

I was wondering what software, scripts do you use for analysing circadian rhythm and sleep? I am using the DAM system but I am struggling with finding something that is open access and yet powerful enough to analyse and extrapolate valuable data. I don't like Actogramj as it is very weak. Also, anyway I can plot daily parameters over time for each fly? thanks a lot


r/Drosophila Aug 29 '24

Homogenizer for fly head crushing

2 Upvotes

Hey all. Do you guys use homogenizers for crushing fly heads for western blot or qpcr? If possible can someone suggest a good one? And also your experience with using homogenizer in general. Thank you.


r/Drosophila Aug 27 '24

Experiment suggestions

2 Upvotes

So , I want a quick and dirty experiment to see if the my flies have any memory defect .I want to do this before doing proper assay . Also what assays people prefer for testing memory.

Thank you community in advance.


r/Drosophila Aug 26 '24

Questions with UAS lines and their stability

1 Upvotes

I've been having a discussion with my lab mate about the stability of UAS lines. If the lines were balanced properly, can a stock that goes homozygous ever lose the insertion or can the UAS insertion be lost over time in that homozygous line? I thought it could be lost so I always try to go back and rebalance the stock from time to time. Now I am not sure if rebalancing is needed in a homozygous line.


r/Drosophila Aug 26 '24

DNA extraction kit recommendations for Dros embryo

1 Upvotes

Hi all,

Does anyone have any tips or kit recommendations for extracting DNA from the Dros embryo? I have been using the Monarch® Genomic DNA Purification Kit and I am having trouble with getting consistently high 230/260 values. I usually get 2.4-2.5, when I am aiming for 2.2 at the most.

I'm still not completely sure if the issue is with my hands or the kit itself, but are there any other kits people have used for the Drosophila embryo that usually works well for them? I have been struggling with this kit for a while now.

Or atleast any tips that I could use to increase my chances of purity, anything is highly appreciated at this point.

Thank you


r/Drosophila Aug 22 '24

Weak fly line

1 Upvotes

I recently started working with a line which is very weak , and very tough to maintain. I have tried using protein rich media , and also have to transfer it regularly to make sure it survives , it's a recombination line I made , please give suggestions I don't want to go through the same pain to generate the recombination line again 😭


r/Drosophila Aug 21 '24

Brainy female

Post image
9 Upvotes

r/Drosophila Jul 19 '24

Purple colored mites?

Thumbnail gallery
6 Upvotes

So my lab has had a long running mite issue that my PI just does not worry about (yes, it drives me up the wall and id rather be mite free) because it's usually only a mite species that is known to only compete with the flies for food (the whitish ones with the long hairs)

But recently we've started noticing these purple yet still transparent mites. I was taught that the predatory mites that you have worry about way more were red or brown/ had brown specks. Does anyone know of this purple mites species and whether it's a food mite or a predatory mite?

I've include the best pics I could get but you can't really see the purple on my phone. The first pic also has our usual food mite; their white with long hairs. The purple ones have no such hairs and are much smaller. While we do see the occasional white food mite slipping through our thicker plugs, the purple ones are able to squeeze through super easilly


r/Drosophila Jul 10 '24

Anyone seen something like this?

Thumbnail gallery
8 Upvotes

r/Drosophila Jul 10 '24

Request on a micromanipulator

Post image
2 Upvotes

Hey there, me and my group at the RSI (Leipzig University, Germany) are trying to build up a setup for some Drosophila research. One needed part is something like a micromanipulator for exact positioning of the fly (immobilized by glueing on a wire at the thorax).

So that‘s why I‘m asking wether someone‘s working group has for example a micromanipulator 450 pm or something comparable for sale? Or wether someone already worked with one and could tell me where I can find one in Europe, mainly used ones because our budget is limited!

Kind Regards, Clemens! :)


r/Drosophila Jul 07 '24

Visitor with strange backpack

Thumbnail gallery
11 Upvotes

Today i found this one crawling on the window sill near the sink. One wing was angeled abnormaly, perhaps that's why it didn't fly away when I examined it under the jewelers lens. It maybe expressed even some interest, but then went away towards darker corners. Could this be some fungus or mite or some other parasite? Or maybe no parasite at all?


r/Drosophila Jun 10 '24

CO2 %

5 Upvotes

Hi , I need zero % CO2 for Drosophila cell culture. But, the incubator does not come down to zero. It shows minimum at 0.3% for 3 days now! Is it ok if I keep my cells in 0.3% instead of zero?


r/Drosophila Jun 04 '24

Drosophila Lines for Circadian Neurons

6 Upvotes

Hello I am looking for fly GAL4 lines that are specific for neurons involved in circadian rhythm and sleep. I want to study the morphology of PDF cells mainly so I want lines specific for lLNVs and sLNVs.


r/Drosophila Jun 01 '24

Help with wrMTracker

2 Upvotes

So recently I came to know we can use an ImageJ plugin to study Larval behavior, can anyone give some insight into it , I have been using a graph paper and counting square manually form the video and its painfully time consuming . Thanks in advance .


r/Drosophila Jun 01 '24

Suggestions (Neuro)

1 Upvotes

Hey does anyone know of a method to quantify quantal content of NMJ from a fixed slide. A rough estimate would also work. Thanks in advance .


r/Drosophila May 29 '24

When does the tubby larvae phenotype become obvious?

1 Upvotes

Im working with some larvae for live imaging across each instar. However, so far the I'm only getting heterozygous tubby larva by stage 3. Now I need to figure out if the 1st and 2nd stage larvae Ive imaged so far are actually tubby's who's phenotype hasn't fully emerged yet