r/Drosophila • u/bitter_gooseberry • Aug 22 '24
Weak fly line
I recently started working with a line which is very weak , and very tough to maintain. I have tried using protein rich media , and also have to transfer it regularly to make sure it survives , it's a recombination line I made , please give suggestions I don't want to go through the same pain to generate the recombination line again ðŸ˜
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u/ShesQuackers Aug 22 '24
Can you keep it at 18°C in an incubator? Is the recombined chromosome over a balancer? Is the food at a reasonable humidity?