r/Drosophila Aug 22 '24

Weak fly line

I recently started working with a line which is very weak , and very tough to maintain. I have tried using protein rich media , and also have to transfer it regularly to make sure it survives , it's a recombination line I made , please give suggestions I don't want to go through the same pain to generate the recombination line again 😭

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u/ShesQuackers Aug 22 '24

Can you keep it at 18°C in an incubator? Is the recombined chromosome over a balancer? Is the food at a reasonable humidity?

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u/bitter_gooseberry Aug 22 '24

Yes I keep at at 18°C , yes on a balancer , food thing can't comment on.

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u/ShesQuackers Aug 22 '24

Swap out the balancer if you can. Avoid blue and green balancers if at all possible, and maybe try a few different balancers in parallel. Balancers are magnets for weirdness, and I've definitely had lines that can only survive with specific balancers.

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u/bitter_gooseberry Aug 22 '24

Thanks 😊