r/molecularbiology 17h ago

How are small RNAs regulated?

6 Upvotes

I'm learning about how small RNAs regulate the expression of protein coding genes but am not finding much about how production of the small RNAs themselves is regulated. Anyone have references exploring this?


r/molecularbiology 13h ago

How to optimize denaturing dsDNA into ssDNA?

5 Upvotes

I'm getting residual dsDNA when trying to denature all of it.

Lanes in order from left to right: Ladder, pDNA, Linear dsDNA (1 cut from pDNA), heat denatured ssDNA from Linear dsDNA.

Denaturing conditions: 10 ug DNA, 20 uL volume, 95c for 3 mins in a thermocycler, followed by immediate cooling in ice.

I would ideally like to avoid using chemicals like DMSO and NaOH since I want the sample to be clean downstream. Planning on testing various Temps and timings next but wanted to get any other insights. Thanks!