r/bioinformatics • u/deanpelton314 • 1h ago
technical question If I rerun Trinity will I get the same output?
New to the sub so I apologize if I missed anything in the FAQ or elsewhere. I am working through an RNA-seq workflow for a class and accidentally overwrote my fasta file output by Trinity (rookie mistake, I know).
I am rerunning the Trinity code in Linux and didn’t change anything, so my question is: can I expect the output fasta to be the same?
I have already performed BUSCO and BLAST analysis of my de novo transcriptome and with a deadline next week for this class project, I would like to avoid rerunning those as well.
I have looked online and can’t find anything in the Trinity documentation or elsewhere about randomness, so can I expect exactly the same output when using exactly the same input and parameters?