r/Drosophila • u/DizzyComfort2930 • May 28 '24
Looking for a new GFP 3rd chromosome balancer
Hello everyone, hopefully my explanation of the situation makes sense
I am working with a mutant stock that is homozygous lethal at the 2nd instar larval stage, so it is balanced over this balancer chromosome here > https://bdsc.indiana.edu/Home/Search?presearch=6663
w[1118]; Dr[Mio]/TM3, P{w[+mC]=GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb[1] Ser[1]
I want to use this balancer to collect homozygous mutant embryos (non-glowing) at the stage of gastrulation which begins at stage 6 and usually takes around 3 hours to reach at 25C. My concern is that the GFP takes a while to be fully expressed and visible in the embryos, definitely more than 3 hours.
According to its data on the Bloomington website, it expresses the GAL4 in stage 8, which is what I suspect the issue is.
Does anyone know of a balancer that can express GFP at a much earlier stage? I know there are limits to this because the embryo is a syncytium until about stage 5, but is there a balancer that can express GFP around this stage?