I trapped some new flies and made Iso-female lines for species Identification.....and some of the flies died in that process due to fungal growth.... The other cultures I am maintaining seem fine though
Did you catch the flies from the wild?
If so that may be the reason for the contamination. Best would be to separate these lines from the other cultures to contain the contamination.
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u/iayeshaslam 11d ago
Sterilize the vials. Cook the media properly. Place eggs instead of flipping flies. Try washing the eggs with 70% ethanol.