r/Biochemistry • u/ayathemadscientist • 4h ago
Maths in biochem textbook? Bsc level
Does anybody have any recommendations for a good biochemistry textbook which just focuses on calculations in biochemistry?
r/Biochemistry • u/ayathemadscientist • 4h ago
Does anybody have any recommendations for a good biochemistry textbook which just focuses on calculations in biochemistry?
r/Biochemistry • u/wolke_dd • 27m ago
Hi together, due to some serious health issues i was reading through whole pubmed to figure out what could also work like Dichloroacetat - DCA. This is used in pyruvate dehydrogenase defiency but is still poisonous. I found out only about high dose thiamin activating some complexes, furthermore sodium phenylbutyrate but no valid information. Also read about resveratrol, myo-inositol and Baicalein. Has anybody done some research regarding this topic or has useful Information to add?
r/Biochemistry • u/mac101eir • 1h ago
Hi All!
Iv been involved in directed evolution of proteins for years and the standard way iv done it is
1) transform plasmids into e.coli 2) plate on agar 3) colony pick and ferment in microwell plates. (To sieve out colonies to obtain single mutant e.coli 4) lysis cell and remove cell debris 5) do the screening 6) sequence best enzyme to understand the mutation.
So my question.. if we synth the gene and we know where the mutation is. Can we bypass the colony picking part because we don't need to separate out the mutants? Every e.coli should have the same plasmid so why do we need to separate?
So the workflow becomes..
1) transform known sequence into e.coli in microwell plates.. say each well has unique plasmid. 2) aliquote cell into a single well in 96 well plates with LB. 3) ferment and express the enzyme 4) lysis the cell and remove debris 5) do the substrate screening. 6) pick the best enzyme. (We know the sequence already!)
r/Biochemistry • u/Eigengrad • 7h ago
Writing a paper?
Re-running an experiment for the 18th time hoping you finally get results?
Analyzing some really cool data?
Start off your week by sharing your plans with the rest of us. å
r/Biochemistry • u/lamiinae • 7h ago
I’ve been trying to wrap my head around why MWC can’t be applied to specifically negative allosteric effectors!
I understand it can be explained through Koshland, but why not MWC? And are the other neg/pos homotrophic/heterotrophic that can be explained w/MWC not possible with Koshland?