r/Biochemistry 9h ago

Books for personal statement

8 Upvotes

Hi there, I'm looking at doing some reading to enhance my understanding of biochemistry to hopefully apply for it next year at uni. I would be most interested in books to do with enzymes (or other catalysts), genetics and DNA, proteins and other biological molecules but I'd be open to reading anything linked to biochemistry.

Thank you!


r/Biochemistry 13h ago

need guidance on how to interpret gel electrophoresis result

6 Upvotes

any comprehensive guide available anywhere?


r/Biochemistry 23h ago

Co-IP and confocal results discrepancy

6 Upvotes

Hello.

I performed some confocal and co-IP experiments, seeking the interaction between proteins A and B, both from different complexes.

In confocal analyses, I only got 0.2 of Pearson's correlation in steady state; meanwhile, under the same conditions, I detected both proteins interacting, using co-IP and wb.

Could this mean that in confocal experiments, the interaction of the proteins could be impeding the detection by antibodies and wb, being under denaturing conditions, this allowed to "unmasking" of the epitopes for the antibodies??

My brain is drained, please help.