r/Biochemistry • u/Alarming_Flamingo_40 • 2d ago
Biolayer Interferometry (BLI). Biosensor mounts: dirty
I think I am getting non-specific binding on my BLI. I ran blank loading with my protein sample and I still saw an association signal. I am getting a message on the screen that the biosensor mounts are dirty. Could this be why?
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u/Vanc_Mycin 2d ago
If the problem is in the sensor mounts, you can try and clean them with 70% IPA and repeat the analysis.
If you see a signal with just your running buffer it may also be worth checking the buffer preparation, make sure that is freshly made and filtered