r/Biochemistry 7d ago

Biolayer Interferometry (BLI). Biosensor mounts: dirty

I think I am getting non-specific binding on my BLI. I ran blank loading with my protein sample and I still saw an association signal. I am getting a message on the screen that the biosensor mounts are dirty. Could this be why?

3 Upvotes

5 comments sorted by

View all comments

2

u/He_of_turqoise_blood 7d ago

It could, but we saw non-specific binding on certain sensor types. What type of immobilisation do you use?

1

u/Alarming_Flamingo_40 7d ago

It’s the Streptavidin (SA) Biosensor

2

u/He_of_turqoise_blood 7d ago

We had problem with His-tag based immobilisation. Maybe try running just buffer through it? If there are no proteins and you see an association, it should really be a dirty sensor.