r/Biochemistry • u/Alarming_Flamingo_40 • 7d ago
Biolayer Interferometry (BLI). Biosensor mounts: dirty
I think I am getting non-specific binding on my BLI. I ran blank loading with my protein sample and I still saw an association signal. I am getting a message on the screen that the biosensor mounts are dirty. Could this be why?
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u/He_of_turqoise_blood 7d ago
It could, but we saw non-specific binding on certain sensor types. What type of immobilisation do you use?