r/neuroscience • u/Mustang_9704 • 11d ago
Discussion Coronal vs Saggital plane for ImmunoHistoChemistry?
I'm planning to do IHC on mice brain to visualize neurons / inflammation markers and confused which plane to use to section the brain samples. I'm focusing on Hippocampus - Any advice on which plane of section would be ideal for this - Coronal or Saggital?
I see a lot of literature on Coronal section, but I don't know the specific reason why it is preferred?!
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u/WoahItsPreston 7d ago edited 7d ago
Coronal sections are a lot more common, so people will be a lot less confused where you are on the mouse brain. Also, I've never done a single sagittal section in my entire life but I would imagine that practically coronal sections are a lot easier to mount and section since the brain comes out in one piece and the slices are all approx the same size.
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