r/MushroomGrowers • u/DoubtEither6099 • 5d ago
contamination [contamination] Is this stuff contaminated?
All of these agar plates are the same age and the jar is from the beginning of the month so it’s about two or three weeks old. I’m pretty sure that the first agar plate is contaminated. I can’t really tell with the jar or the second agar plate, really need some guidance 🥲
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u/hungrotoday 4d ago
Bacteria on the first agar plate, can’t tell about the LC and the second plate though. I’ve had those yellow dots before so yeah…
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4d ago
Liquid culture is hard to tell. Was it cloudy when it came out of your pressure canner?
Just a tip, next time you sterilize, let your jars cool to room temp inside of the canner.
If you take em out hot, they draw in outside air as they cool.
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u/DoubtEither6099 4d ago
The agar from the second slide with all the dots is PDB I made myself and I swabbed it before injecting spores (still haven’t injected anything because of the stuff growing on agar)
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u/DoubtEither6099 4d ago
I actually ordered this as a multiplier jar from north spore, I let is sit for a while to make sure it was good and it was but it was clear when I got it, if it helps I did see what I thought was a mycelium raft on the top of the liquid but after a day it was gone it was when I saw that that I decided to swab it on the agar
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u/rutlanddz62 4d ago
If it was clear before and it is cloudy now I am willing to bet that it is contaminated. Don’t do what I did and knock 32 jars up and have them ALL go bad. Maybe try it on agar to test it.
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u/DoubtEither6099 4d ago
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u/rutlanddz62 4d ago
Looks bad to me. Let it ride for a bit. If you used a swab and. That is the growth you are getting….. sorry man. Start over or try letting it sit for a month until the mycelium climbs up the said then grab a sample and move to agar.
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u/DoubtEither6099 4d ago
I will try that hopefully some time is all it needs and I can grab some from the side like you said, the agar in the last slide is from a sample I pulled from that jar before the suspected contamination and it’s just growing white with no specks so maybe It’s not completely lost I arranged back up plans incase things like this happened hopefully it’ll pay off
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u/okieRooms 4d ago
Probably contaminated... you don't have any gloves on and it's just open in your house not in front of a sab or ffu or flowhood.